Retrieved from ” https: This page was last edited on 17 February , at Nov 27, 6. Generally, Scatchard and Lineweaver-Burk plots are outdated. This article may be too technical for most readers to understand. Any help would be greatly appreciated! It turns out that the answer was 6. Wikipedia articles needing context from October All Wikipedia articles needing context Wikipedia introduction cleanup from October All pages needing cleanup Articles needing sections from October All articles needing sections Articles covered by WikiProject Wikify from October All articles covered by WikiProject Wikify Wikipedia articles that are too technical from October All articles that are too technical Articles needing expert attention from October All articles needing expert attention Articles with multiple maintenance issues.
Please help improve the article with a good introductory style. Anyone know what I did wrong? Yes, my password is: This biochemistry article is a stub. The plot yields a straight line of slope – K , where K is the affinity constant for ligand binding. Nov 28, 8. I’m probably in your class UCD MCB We can help each other out if you like, but I sort of don’t do my homework until the last minute, as you can see. In a Scatchard plot, assumptions of independence in linear regression model is violated because B bound ligand is used in the X and Y axes.
Any help would be greatly appreciated!
pllot Anyone know what I did wrong? Relative binding affinities between two sites can be distinguished with a line showing identical affinity and a curve showing different affinities. From Wikipedia, the free encyclopedia. In a Scatchard plot, assumptions of independence in linear regression model is violated because B bound ligand is used in the X and Y axes.
Eadie Scatchard Plot | Physics Forums
Nov 27, 6. This biochemistry article is a stub. Like I just thought it would be easier to plot the values without all the extra zeros.
But don’t forget to label figs with what they are, and the units as well as numbers. Therefore, the typical measure of goodness of fit for linear regressionthe correlation coefficient Ris not scahchard. Learn how and when to remove these template messages.
Scatchard equation – Wikipedia
But i think you have to look at your units again. You just copy what it gives you and paste it here and it comes up – there used to be a delay here for checks but I think there isn’t any more.
Their original intention was to transform the data into linear representations of the original data such that linear regression methods could be applied.
Nov 25, 4.
BorekNov 25, Now I got a linear regression line that actually makes sense. Please help improve the article with a good introductory style. Nov 29, This article may be too technical for most readers to understand. Everyone who loves science is eadid No, create an account now.
One drawback from the Eadie—Hofstee approach is that neither ordinate nor abscissa represent independent variables: Please help improve it to make it understandable to non-expertswithout removing the technical details.
Now take uMol and divide it by 10uL; 10uL because that’s how much you extracted in the experiment. Nov 24, 1. It is named after the American chemist George Scatchard  and is sometimes referred to as the Rosenthal-Scatchard equation. Thus any experimental error will be present in both axes.
Retrieved from ” https: Nov 28, 7. The Scatchard equation is an equation used in molecular biology for calculating the affinity constant of a ligand with a protein. The plot yields a straight line of slope – Kwhere K is the affinity constant for ligand binding. I checked my math and I got 6 binding sites too.